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Whole Transcriptome Sequencing | Total RNA & Expression Test | Henotic Diagnostics
Whole Transcriptome Sequencing: Fast & Precise Total RNA Expression Test
Advanced Clinical Whole Transcriptome Sequencing & Total RNA Experts
Clinical Overview & Diagnostic Indications
First of all, Whole RNA Sequencing is the gold-standard molecular assay that analyzes the entire spectrum of expressed RNA transcripts, including messenger RNA (mRNA) and non-coding RNA (lncRNA, miRNA). Essentially, high-depth Next-Generation Sequencing profiles dynamic gene expression, oncogenic gene fusions, and alternative splice variants with 99.9% accuracy. Consequently, oncologists and clinical geneticists uncover critical diagnostic biomarkers that standard DNA testing misses.
Furthermore, book your Whole RNA Sequencing at Henotic Diagnostics for complete transcriptomic clarity. In addition, our accredited laboratory operates 24/7 with specialized RNA sample logistics support.
To begin with, Whole RNA Sequencing captures the entire catalog of active RNA transcripts within a tissue or cell population. At the same time, experts at Henotic Diagnostics deploy total RNA depletion pipelines to sequence both polyadenylated mRNA and regulatory non-coding RNA species simultaneously.
What Does Whole Transcriptome Sequencing Evaluate?
Specifically, Whole RNA Sequencing quantifies relative expression levels across thousands of active genes to build comprehensive transcriptional profiles. Consequently, bioinformatic algorithms detect actionable driver fusions (NTRK, RET, ALK, ROS1, BRAF), novel splice isoforms, and long non-coding RNA (lncRNA) dysregulation. Furthermore, high-depth sequencing uncovers allele-specific expression patterns. As a result, physicians prescribe highly targeted precision therapies. In fact, to review foundational transcriptomic research, consult the NIH NHGRI Transcriptome Fact Sheet.
Furthermore, Advanced Tech Sequencing Machine ensuring a smooth, RNA expression and fusion target insights.
What is Whole Transcriptome Sequencing?
In general, while Whole Exome DNA Sequencing reveals static genomic variants, Whole RNA Sequencing profiles dynamic gene expression activity. Essentially, total cellular RNA is extracted, depleted of abundant ribosomal RNA, converted into cDNA, and sequenced on high-throughput NGS platforms. Therefore, bioinformatic software maps millions of sequence reads to quantify exact transcript expression. Additionally, to read about technological advances, examine the Nature Biotechnology journal.
Additionally, high-throughput NGS platforms sequence total cellular RNA with 99.9% diagnostic precision
What Does Whole Transcriptome Sequencing Check?
Overall, clinical Whole RNA profiling screens across multiple molecular categories:
Key RNA & Transcriptomic Biomarkers Evaluated
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Coding Messenger RNA (mRNA) Expression: First, measures absolute and relative transcription levels across all protein-coding genes.
Non-Coding RNA Networks (lncRNA & miRNA): In addition, profiles regulatory long non-coding and microRNA expression. Review the NCBI Gene Expression Omnibus repository.
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Alternative Splice Variants: Finally, identifies abnormal exon skipping, intron retention, and mutant isoform expression.
In addition, bioinformatic software maps whole transcriptome expression heatmaps, fusion junctions, and splice graphs
When Is Whole Transcriptome Sequencing Recommended?
For example, oncologists order Whole RNA Sequencing for advanced, metastatic, or treatment-resistant solid tumors to find actionable gene fusions. In particular, testing is recommended for lung carcinoma, soft tissue sarcomas, thyroid carcinoma, and pediatric malignancies. Moreover, it is used in rare genetic disease research when DNA sequencing fails to identify pathogenic mechanisms. In addition, to verify precision medicine protocols, examine the NCI Precision Medicine in Cancer guide.
Moreover, expert clinical consultation translates whole transcriptome fusion data into targeted drug therapies
Clinical Superiority: Whole Transcriptome (WTS) vs. Targeted RNA Panels
Ideally, clinicians should order Whole RNA Sequencing when targeted RNA panels fail to identify driver mutations. In addition, targeted RNA panels query only known 3′ and 5′ fusion partners, whereas WTS captures untargeted novel fusion breakpoints across the entire genome. Indeed, to review global genomic health facts, consult the WHO Genomic Diagnostics Fact Sheet.
Whole Transcriptome Sequencing Cost Guide
Clinical WTS Service
Approximate Cost (₹)
Whole Transcriptome RNA-Seq (mRNA + lncRNA)
First, whole transcriptome sequencing pricing is ₹45,000 – ₹58,000.
MBBS, MD (Genomics) · 15+ Years Clinical Experience
Furthermore, your Whole Transcriptome Sequencing data is interpreted by accredited molecular geneticists and bioinformaticians. Consequently, comprehensive reports empower oncologists with actionable RNA expression and fusion target insights.
Our Accreditations & Quality Standards
Certified excellence ensuring total RNA sequencing depth, bioinformatic fusion calling accuracy, and patient privacy.
What Our Patients Say
Real feedback from patients who completed Whole Transcriptome Sequencing with us.
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“Whole Transcriptome Sequencing identified an unknown RET fusion in my biopsy sample. Furthermore, targeted therapy began immediately with remarkable response!”
– Arvind B.
Verified Patient Review
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“Exceptional sample handling and PaxGene tube logistics. In addition, the clinical bioinformatician answered all our physician’s questions.”
– Radhika S.
Verified Patient Review
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“Premier NABL laboratory accuracy. Henotic Diagnostics provides state-of-the-art total RNA sequencing for complex oncology cases.”
If you require Whole Transcriptome Sequencing for total RNA expression or oncogenic fusion detection, do not delay. Call to schedule your sample collection.
Henotic Diagnostics
Second floor, Millennium Empire, Business Park, Plot No 47, D Mart Rd, Sector 15, Kharghar, Navi Mumbai, Panvel, Maharashtra 410210
Common Questions About Whole Transcriptome Sequencing
You can book your Whole Transcriptome Sequencing appointment at our Navi Mumbai center through multiple convenient channels: call us directly at 088793 27184, send a WhatsApp message for instant booking, or use the online booking form on our website. Walk-in appointments are also welcome based on slot availability. We recommend advance booking to ensure zero wait times.
Yes, Henotic Diagnostics Navi Mumbai is empanelled with all major insurance companies and TPAs (Third Party Administrators) including Star Health, ICICI Lombard, New India Assurance, HDFC Ergo, Max Bupa, Bajaj Allianz, and many more. We offer cashless processing for Whole Transcriptome Sequencing where applicable. Please carry your insurance card and a valid ID for seamless processing.
At Henotic Diagnostics Navi Mumbai, most Whole Transcriptome Sequencing reports are delivered within 2-6 hours for routine tests. Critical and urgent reports are expedited on a priority basis. All reports are delivered digitally via WhatsApp, email, and our secure online patient portal. Hard copies are also available at the center upon request. You will receive an SMS/WhatsApp notification the moment your report is ready.
Henotic Diagnostics Navi Mumbai operates 24 hours a day, 7 days a week, including Sundays and public holidays. This means you can schedule your Whole Transcriptome Sequencing at a time that is most convenient for you — early morning, late evening, or even overnight for emergency requirements. Our radiology and pathology departments maintain round-the-clock availability.
Yes, Henotic Diagnostics Navi Mumbai is fully accredited by NABL (National Accreditation Board for Testing and Calibration Laboratories) and ISO 9001:2015 certified. Our radiology equipment also complies with AERB (Atomic Energy Regulatory Board) safety standards. These accreditations ensure that every Whole Transcriptome Sequencing performed at our facility meets the highest national and international quality benchmarks for accuracy, safety, and reliability.
