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16s Rrna Sequencing | Bacterial Microbiome Test | Henotic Diagnostics
16s Rrna Sequencing: Fast & Precise Bacterial Microbiome Test
Advanced Bacterial 16s Rrna Sequencing Experts
First of all, 16s Rrna Sequencing (16S Ribosomal RNA Gene Sequencing) is the gold standard Next-Generation Sequencing (NGS) technique for profiling bacterial communities. Essentially, amplifying hypervariable V3-V4 gene regions identifies gut bacterial species, taxonomic diversity, phylum ratios, and metabolic dysbiosis with 99.9% accuracy. As a result, clinicians treat IBS, SIBO, autoimmune inflammation, and metabolic disorders effectively.
Book your 16S Amplicon Sequencing at Henotic Diagnostics for comprehensive microbiome insight. Furthermore, our accredited diagnostic facility operates 24/7 with convenient home sample collection services.
To begin with, 16S Amplicon Sequencing evaluates the ribosomal RNA gene present in all prokaryotic bacterial genomes. At the same time, experts at Henotic Diagnostics sequence stool DNA to map hundreds of bacterial genera inhabiting your gastrointestinal tract.
What Does 16s Rrna Sequencing Evaluate?
Specifically, 16S amplicon sequencing targets Nine hypervariable regions (V1-V9), with V3-V4 providing optimal taxonomic resolution for genus and species identification. Consequently, bioinformatic algorithms profile beneficial probiotic strains (Bifidobacterium, Lactobacillus, Akkermansia) alongside opportunistic pathogens (Clostridium, Enterobacter). Furthermore, high-depth NGS measures alpha and beta diversity metrics. As a result, gastroenterologists craft targeted dietary and probiotic therapies. In fact, to review international microbiome research standards, consult the NIH Human Microbiome Project resource.
Furthermore, comfortable reception lounge providing discreet intake for stool DNA collection kits
What is 16s Rrna Sequencing?
In general, the 16S rRNA gene contains highly conserved regions interspersed with nine hypervariable regions unique to bacterial species. Essentially, universal PCR primers bind conserved sequences to amplify variable regions. Specifically, Next-Generation Sequencers generate millions of sequence reads which are matched against curated databases like SILVA or Greengenes. Therefore, 16S sequencing provides an accurate bacterial census without needing live bacterial culture. Additionally, this test pinpoints silent bacterial dysbiosis. In fact, to review international microbiome research standards, consult the NIH Human Microbiome Project resource.
Pathobiont Expansion: In addition, detects pro-inflammatory bacterial overgrowth associated with leaky gut syndrome and IBS.
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Alpha & Beta Diversity Score: Finally, computes Shannon and Simpson biodiversity indices to score overall ecosystem resilience.
In addition, bioinformatic software maps bacterial taxonomic trees and phylum abundance charts
When Is 16s Rrna Sequencing Recommended?
For example, gastroenterologists, functional medicine doctors, and clinical nutritionists recommend 16S Amplicon Sequencing for chronic digestive issues. In particular, testing is advised for patients experiencing persistent bloating, Irritable Bowel Syndrome (IBS), or Small Intestinal Bacterial Overgrowth (SIBO). Moreover, it is recommended following long-term antibiotic therapy to measure gut flora recovery. In addition, individuals dealing with autoimmune skin conditions or metabolic fatigue order 16S sequencing for targeted probiotic restoration. In fact, to review international microbiome research standards, consult the NIH Human Microbiome Project resource.
Moreover, expert consultation translates complex 16S bacterial data into personalized gut health plans
Clinical Distinction: 16S rRNA Amplicon vs. Stool Bacterial Culture
Why 16S DNA Sequencing Outperforms Traditional Stool Culture
Ideally, patients should choose 16S Amplicon Sequencing if traditional stool culture tests return normal despite chronic symptoms. In addition, over 85% of gut bacteria are obligate anaerobes that cannot grow on petri dishes. Indeed, 16S DNA sequencing detects non-culturable bacteria with complete precision. In fact, to review international microbiome research standards, consult the NIH Human Microbiome Project resource.
16s Rrna Sequencing Cost Guide
Diagnostic Metagenomic Service
Approximate Cost (₹)
16S rRNA V3-V4 Bacterial Profiling Panel
First, standard 16S sequencing costs ₹12,500 – ₹16,500.
In addition, home collection service is complimentary.
Clinical Bioinformatic Report Consultation
Finally, expert consultation fee is ₹1,000 – ₹1,500.
Comparison: 16S Amplicon Sequencing vs. Shotgun Metagenomics
On one hand, Shotgun Metagenomic sequencing reads all genomic DNA (bacterial, viral, fungal, human), whereas 16S Amplicon Sequencing specifically targets bacterial taxonomy at a lower cost. Consequently, 16S sequencing is the most cost-effective tool for routine bacterial dysbiosis screening.
MBBS, MD (Microbial Bioinformatics) · 15+ Years Clinical Experience
Furthermore, your 16s Rrna Sequencing data is processed through accredited bioinformatic pipelines aligning reads against the SILVA database. Consequently, detailed reports empower gastroenterologists with actionable bacterial insights.
Our Accreditations & Quality Standards
Certified excellence ensuring amplicon sequencing depth, taxonomic alignment accuracy, and patient data privacy.
What Our Patients Say
Real feedback from patients who completed 16S rRNA microbiome profiling with us.
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“After suffering from chronic bloating for 2 years, 16s Rrna Sequencing identified severe Akkermansia deficiency. Furthermore, targeted prebiotic therapy resolved my symptoms!”
– Vikram S.
Verified Patient Review
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“The stool collection kit arrived directly at home with simple instructions. In addition, the bioinformatic report was easy to discuss with my gastroenterologist.”
You can book your 16s Rrna Sequencing appointment at our Navi Mumbai center through multiple convenient channels: call us directly at 088793 27184, send a WhatsApp message for instant booking, or use the online booking form on our website. Walk-in appointments are also welcome based on slot availability. We recommend advance booking to ensure zero wait times.
Yes, Henotic Diagnostics Navi Mumbai is empanelled with all major insurance companies and TPAs (Third Party Administrators) including Star Health, ICICI Lombard, New India Assurance, HDFC Ergo, Max Bupa, Bajaj Allianz, and many more. We offer cashless processing for 16s Rrna Sequencing where applicable. Please carry your insurance card and a valid ID for seamless processing.
At Henotic Diagnostics Navi Mumbai, most 16s Rrna Sequencing reports are delivered within 2-6 hours for routine tests. Critical and urgent reports are expedited on a priority basis. All reports are delivered digitally via WhatsApp, email, and our secure online patient portal. Hard copies are also available at the center upon request. You will receive an SMS/WhatsApp notification the moment your report is ready.
Henotic Diagnostics Navi Mumbai operates 24 hours a day, 7 days a week, including Sundays and public holidays. This means you can schedule your 16s Rrna Sequencing at a time that is most convenient for you — early morning, late evening, or even overnight for emergency requirements. Our radiology and pathology departments maintain round-the-clock availability.
Yes, Henotic Diagnostics Navi Mumbai is fully accredited by NABL (National Accreditation Board for Testing and Calibration Laboratories) and ISO 9001:2015 certified. Our radiology equipment also complies with AERB (Atomic Energy Regulatory Board) safety standards. These accreditations ensure that every 16s Rrna Sequencing performed at our facility meets the highest national and international quality benchmarks for accuracy, safety, and reliability.
